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RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Combinatorial library screening for developing an improved split-firefly luciferase fragment-assisted complementation system for studying protein–protein interactions. Binding proteins selected from combinatorial libraries of an α-helical bacterial receptor domain. Connection between absorption properties and conformational changes in Deinococcus radiodurans phytochrome. Takala, H., Lehtivuori, H., Hammarén, H., Hytönen, V. Mutational analysis of Deinococcus radiodurans bacteriophytochrome reveals key amino acids necessary for the photochromicity and proton exchange cycle of phytochromes. Signal amplification and transduction in phytochrome photosensors. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Engineering a far-red light-activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors. A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice. Biosynthesis of chromophores for phytochrome and related photoreceptors. A small and highly sensitive red/far-red optogenetic switch for applications in mammals. A phytochrome sensory domain permits receptor activation by red light. Reichhart, E., Ingles-Prieto, A., Tichy, A. Spatiotemporal control of cell signalling using a light-switchable protein interaction. A clearer vision for in vivo imaging biological imaging. A photoactivatable botulinum neurotoxin for inducible control of neurotransmission. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Evolution of a split RNA polymerase as a versatile biosensor platform. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. A photoactivatable Cre- loxP recombination system for optogenetic genome engineering. A split CRISPR–Cpf1 platform for inducible genome editing and gene activation.
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Photoactivatable CRISPR–Cas9 for optogenetic genome editing. CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation. CRISPR–Cas9-based photoactivatable transcription system. Nihongaki, Y., Yamamoto, S., Kawano, F., Suzuki, H. Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation.
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Optogenetic control of intracellular signaling pathways. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Rapid blue-light-mediated induction of protein interactions in living cells.